Oct 13, 2015
It is always good to know how to use any of your equipment appropriately. This is essential not only to get efficient performance but most importantly to achieve maximum safety.
Watch the following video on the things that you need to do and not to do when using Laminar Flow Cabinets. Enjoy watching!
Always minimize clutter.
Laminar flow cabinets are not storage cabinets. The presence of clutter obstructs the laminar flow around the work area. Furthermore, clutter makes it very difficult to clean the work surface.
Always wash your hands and arms before entering.
Personnel is the most crucial source of contamination, and touching is the most common means of introducing contamination to a sterile product. Proper hand washing is extremely important since the fingers have countless bacterial components. Hand washing should not only be performed before working in the work bench but also after.
Arrange objects in such a way that full benefits of the laminar flow of air can be achieved.
Properly arranging all items in the laminar flow workbench provides a work area that is directly bathed with clean air from the ULPA filter.
Avoid spraying or squirting solutions onto the HEPA/ULPA filter.
Nothing should be permitted to come in contact with the HEPA/ULPA filter to avoid contamination of the air downstream.
Remove outer pouches and wraps on the edge of the work area.
Outer pouches and wraps are non-sterile items, meaning they may introduce contaminants to the work bench. They are also considered as clutter, hence they may disrupt the laminar airflow pattern.
Keep large objects away from the back of the hood.
Large objects contaminate all the other items downstream and also disrupt the laminar airflow pattern which suspends the contaminants and removes them from the area.
Keep coughing, quick movements, and talking to a minimum in the main work area.
Personnel activity generate particles. Particles can disrupt the laminar flow of air, which may also be a possible cause of contamination. If talking is necessary, keep it away from the work bench.
Always disinfect your materials before entering the laminar flow cabinet.
Only sterile materials should be placed inside the clean work area. Make sure that all your items are disinfected properly to avoid possible contamination of the work bench.
Put any waste and other items in the hood.
Waste and non-sterile items create turbulence of airflow and introduce contaminants to the clean work bench and to the sterile samples.
Wear any jewelry around your hands and wrists.
Jewelry are non-sterile items, therefore they introduce bacteria or particles into the clean work area. They also reduce hand cleanliness if they are worn inside the work bench.
Place critical items away from the air source.
Keep a direct open path between the filter and the area inside the hood where work procedures are performed. Air downstream from nonsterile items are contaminated with particles generated from these objects.
Aim near the filter when opening ampoules or adjusting syringes.
Ampoules and syringes contain salt solutions that may be deposited onto the HEPA/ULPA filter, which can damage the filter.
Bring unnecessary items into the main work area.
The introduction of non-essential items into the work area should be avoided whenever possible. Traffic flow into a clean area should be minimized.
Place large objects near the back of the cabinet, because they disrupt the laminar flow pattern of air and contaminate everything downstream.
Use materials that were not disinfected.
Materials that were not disinfected introduce contamination to the clean work bench, therefore defying the primary purpose of a laminar flow cabinet, which is to protect your products from any room contaminants.